Journal: PLoS ONE

Article Title: Development of a Multiplexed Bead-Based Suspension Array for the Detection and Discrimination of Pospiviroid Plant Pathogens

doi: 10.1371/journal.pone.0084743

Figure Lengend Snippet: Predictions of the number of isolates of each pospiviroid species that will be detected using universal and species-specific assays of the Luminex MagPlex-TAG pospiviroid array.

Article Snippet: To prepare the mixture of 11 optically distinct Luminex MagPlex-TAG beads , each bottle of MagPlex-TAG microspheres was vortexed vigorously for 1 min, then sonicated for 30 s. Bead mixtures were prepared in Eppendorf LoBind tubes (Eppendorf, Hamburg, Germany), diluted with 2×Tm hybridization buffer (0.2 M Tris-HCl pH 8.0, 0.4 M NaCl, 0.16% Triton X-100) to yield a final concentration of approximately 1250 beads of each bead address per 25 µL.

Techniques: Luminex

Journal: PLoS ONE

Article Title: Development of a Multiplexed Bead-Based Suspension Array for the Detection and Discrimination of Pospiviroid Plant Pathogens

doi: 10.1371/journal.pone.0084743

Figure Lengend Snippet: Flow diagram depicting the five steps of the Luminex MagPlex-TAG pospiviroid array method.

Article Snippet: To prepare the mixture of 11 optically distinct Luminex MagPlex-TAG beads , each bottle of MagPlex-TAG microspheres was vortexed vigorously for 1 min, then sonicated for 30 s. Bead mixtures were prepared in Eppendorf LoBind tubes (Eppendorf, Hamburg, Germany), diluted with 2×Tm hybridization buffer (0.2 M Tris-HCl pH 8.0, 0.4 M NaCl, 0.16% Triton X-100) to yield a final concentration of approximately 1250 beads of each bead address per 25 µL.

Techniques: Luminex

Journal: PLoS ONE

Article Title: Development of a Multiplexed Bead-Based Suspension Array for the Detection and Discrimination of Pospiviroid Plant Pathogens

doi: 10.1371/journal.pone.0084743

Figure Lengend Snippet: The sensitivity of the multiplexed Luminex MagPlex-TAG pospiviroid array when tested using a ten-fold serial dilutions of a total RNA extract from a Solanum lycopersicum sample infected with Potato spindle tuber viroid (PSTVd, isolate #N).

Article Snippet: To prepare the mixture of 11 optically distinct Luminex MagPlex-TAG beads , each bottle of MagPlex-TAG microspheres was vortexed vigorously for 1 min, then sonicated for 30 s. Bead mixtures were prepared in Eppendorf LoBind tubes (Eppendorf, Hamburg, Germany), diluted with 2×Tm hybridization buffer (0.2 M Tris-HCl pH 8.0, 0.4 M NaCl, 0.16% Triton X-100) to yield a final concentration of approximately 1250 beads of each bead address per 25 µL.

Techniques: Luminex, Infection

Journal: PLoS ONE

Article Title: Development of a Multiplexed Bead-Based Suspension Array for the Detection and Discrimination of Pospiviroid Plant Pathogens

doi: 10.1371/journal.pone.0084743

Figure Lengend Snippet: Percentages of false positive and false negative results for Luminex MagPlex-TAG pospiviroid array data when analyzed using three different methods for setting thresholds for positivity.

Article Snippet: To prepare the mixture of 11 optically distinct Luminex MagPlex-TAG beads , each bottle of MagPlex-TAG microspheres was vortexed vigorously for 1 min, then sonicated for 30 s. Bead mixtures were prepared in Eppendorf LoBind tubes (Eppendorf, Hamburg, Germany), diluted with 2×Tm hybridization buffer (0.2 M Tris-HCl pH 8.0, 0.4 M NaCl, 0.16% Triton X-100) to yield a final concentration of approximately 1250 beads of each bead address per 25 µL.

Techniques: Luminex

Journal: PLoS ONE

Article Title: Development of a Multiplexed Bead-Based Suspension Array for the Detection and Discrimination of Pospiviroid Plant Pathogens

doi: 10.1371/journal.pone.0084743

Figure Lengend Snippet: Details of pospiviroid isolates used for validation of the Luminex MagPlex-TAG pospiviroid array.

Article Snippet: To prepare the mixture of 11 optically distinct Luminex MagPlex-TAG beads , each bottle of MagPlex-TAG microspheres was vortexed vigorously for 1 min, then sonicated for 30 s. Bead mixtures were prepared in Eppendorf LoBind tubes (Eppendorf, Hamburg, Germany), diluted with 2×Tm hybridization buffer (0.2 M Tris-HCl pH 8.0, 0.4 M NaCl, 0.16% Triton X-100) to yield a final concentration of approximately 1250 beads of each bead address per 25 µL.

Techniques: Luminex